QUAREP-LiMi

Overview

QUAREP-LiMi (Quality Assessment and Reproducibility for Instruments & Images in Light Microscopy) is a global community-driven initiative founded in April 2020, comprising 590+ members from 41 countries across academia and industry. It works to establish common quality control (QC) standards, metadata models, guidelines, and tools for light microscopy.

Working Groups (15 active)

Key areas include instrument QC, metadata standards, image quality metrics, fluorescent reference standards, publication guidelines, and AI-compatible data mining. Outputs are being formalised into ISO standards (ISO 172/SC 5 Microscopes and Endoscopes).

Key Outputs

• QUAREP-LiMi produces Standard Operating Procedures for widefield and confocal QC.
• It publishes MIAME-compliant metadata guidelines for light microscopy experiments.
• Its guidelines are being formalised into ISO standards (ISO 172/SC 5 Microscopes and Endoscopes).
• Community resources are hosted at forum.image.sc, an open discussion platform.

Connections

• Collaborates with: OME, Euro-BioImaging
• French counterpart: GT-GeDeM

Resources

• https://quarep.org
• https://www.nature.com/articles/s41592-021-01162-y (Nature Methods 2021)
• https://forum.image.sc (community forum, tagged quarep-limi)